Saturday, June 2, 2012

Optical tweezers help researchers uncover key mechanics in cellular communication

Optical tweezers help researchers uncover key mechanics in cellular communication

Friday, June 1, 2012

By using a laser microbeam technology called optical tweezers, UC Irvine and UCLA researchers have uncovered fundamental properties of a key molecular signaling system involved with development, cancer and cardiovascular disease.

In collaboration, UCI's Elliot Botvinick and UCLA's Gerry Weinmaster published online in the journal Developmental Cell complementary studies in which they each used optical tweezers to detect and measure the mechanical force produced by cells when bound to Notch, a cellular pathway that ensures the correct cell types form at a precise time and location in the body.

"The Notch network is used repeatedly during the development of almost every cell type and must be tightly controlled, as inappropriate communication causes developmental defects and cancer," said Weinmaster, a professor of biological chemistry and researcher at UCLA's Jonsson Comprehensive Cancer Center. "Successful design and generation of Notch therapeutics demands a solid understanding of the basic mechanics of the Notch network."

"Optical tweezers act as tiny tractor beams that can hold and manipulate microscopic beads coated with specific molecules," said Botvinick, an assistant professor of biomedical engineering and surgery affiliated with the Beckman Laser Institute and The Edwards Lifesciences Center for Advanced Cardiovascular Technology at UCI. "When cells bind to and pull on the beads, researchers can measure cell-generated forces that are billions of times smaller than the weight of one teaspoon of sugar."

Using this technology, the UCI-UCLA team found that communication via the Notch network involves a sort of tug-of-war between neighboring cells in which Notch molecules are unraveled by force to reveal hidden elements important for cell-to-cell communication.

Together with biochemical and biological cell analyses, their findings provide compelling evidence that pulling on Notch opens a network to deliver instructions for specific cellular responses.

The research sheds new light on the role of cells' neighbors in the development and regulation of tissue and advances efforts to create new therapeutics.

Bhupinder Shergill of UCI and Laurence Meloty-Kapella, Abdiwahab Musse and Jane Kuon of UCLA contributed to the studies.

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University of California - Irvine: http://www.uci.edu

Thanks to University of California - Irvine for this article.

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US court battle over lethal drugs

The US Food and Drug Administration is going to court to secure supplies of a drug used in lethal injections.

Sodium thiopental is used in many states to anaesthetise prisoners before the administration of other chemicals that extinguish life.

However supplies have dwindled in the US after a judge banned its importation in March.

Campaigners against the death penalty say the restrictions have forced a slowdown in the rate of executions.

The last US manufacturer of sodium thiopental ceased production in 2009. Since then the drug has been imported mainly from companies within the European Union. Several states purchased supplies from a UK based firm called Dream Pharma, run from a driving school in west London.

But these companies have in turn stopped delivering to the US as the European Union cracked down on the export of medicines that can be used in executions.

Legal confusion

In March of this year a US district judge upheld a case brought by 21 prisoners on death row who argued that the FDA didn't have the right to allow prisons to import the drug without ensuring that it would work effectively.

However under pressure from many of the 33 states that use the lethal injection method, the FDA is appealing that ruling. Experts say it is likely to take a couple of months to resolve. And either side could then appeal to the Supreme Court which may take a year to come to a decision.

According to Maya Foa from Reprieve, a group that campaigns against the death penalty, the legal confusion and the shortage of supplies have led to a slowdown in executions.

"There have certainly been shortages which have caused many states to slow the rate of executions. It's also raised the cost - There have been moratoria over the past year and a half while states scramble to find more drugs," she told BBC News.

Richard Dieter, executive director of the US based Death Penalty Information Center, agrees that the legal confusion over the status of sodium thiopental is having some effect on executions.

"It's slowed things down somewhat but the big states have enough supplies to keep going."

One dose left

The state of Oklahoma has the highest rate of executions per capita in the US. It uses a different drug called pentobarbital. This has been used in the treatment of medical disorders including anxiety. But restrictions placed on supplying it for lethal injections by the manufacturers mean that, following the execution of Michael B Selsor at the beginning of May, the state is running low.

Jerry Massie, a spokesman for the Oklahoma department of corrections acknowledged the state has only one dose of pentobarbital left.

"The problem is that companies don't want to sell it to prisons. We're still looking at options but we haven't made a decision on an alternative yet."

One state that has made a decision on an alternative is Missouri. They have decided to use Propofol as an anaesthetic agent in future executions. An overdose of the drug caused the death of pop star Michael Jackson. In a written statement the Missouri department of corrections acknowledged that shortages of other drugs had forced their hand.

"The one-drug protocol replaces the state's previous three-drug protocol. This change became necessary due to the unavailability of sodium thiopental, one of the three drugs used under the previous protocol."

Continue reading the main story

There's a sort of medical veil pulled over executions which prisons and states which are pro capital punishment like, they need that to hide the reality of what is actually a barbaric practice?

End Quote Maya Foa Reprieve

Some critics of the death penalty are concerned that propofol has never been used for executions before. Richard Dieter from the Death Penalty Information Center says that isn't really the issue.

"There's lots of anaesthetics out there, if you give enough of it you can kill someone," he said.

The legal and commercial restrictions on drugs used in executions are not just affecting US states. In July of last year Vietnam decided to switch to lethal injection but despite having more than 400 prisoners on death row, no one has been executed since. Officials in the Ministry of Public Security were quoted as saying that getting supplies of the drugs needed for lethal injections has proved difficult.

Many states have chosen to use the lethal injection because they believe it to be a more humane method than alternatives such as hanging or the electric chair. And according to Maya Foa from Reprieve they will be very reluctant to go back to these methods

"There's a sort of medical veil pulled over executions which prisons and states which are pro capital punishment like, they need that to hide the reality of what is actually a barbaric practice," she told BBC News.

I think they would be unlikely to go back to hanging or electrocution because that forces the public to witness the reality of putting a man to death and that's something that actually repels a lot of people and would increase the anti-capital punishment sentiment out there and that's a risk I think that prisons and states are unwilling to take."

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What Is a Breast Augmentation Really Like?

You are considering a breast augmentation and you are excited about the possibilities it can offer to you but at the same time you are wondering what the procedure and process will really be like. Though each surgeon and surgery is different, most will provide you with a good idea of what you can expect to occur the days before and after your procedure. In most cases, women will have a good outcome where their goals are met. However, it is possible that you may feel pain and discomfort, too. Sometimes, you just want to know what you can expect.

Leading Up to the Surgery

In the days and weeks leading up to the breast augmentation, you will meet with your surgeon to discuss the options and to make some big decisions. You will need to have an exam and, in some cases, you will need to ensure you are healthy. In addition, you will need to talk about the options for your breasts including sizes, texture, filling and placement.

It is common for your surgeon to then consider your overall body's needs and to offer suggestions about what to do before you have your procedure to ensure you are healthy, such as taking an antibiotic to ward off infection.
The Day of the Procedure

The day of the breast augmentation, you will be prepped and prepared for the procedure. You will see your doctor before having the procedure. He or she will discuss your needs and any concerns you have at this time. The procedure will last a few hours at the most. After wards, you will not remember anything because you will be under general anesthesia throughout it. This means you will feel no pain during the process. After wards, you are likely to recover for several hours in the hospital or medical center before being discharged to your home. Some people may need to spend the night in the hospital to recover.

After the Procedure

After you have the procedure, you will need several days to heal and overcome the new strain. This takes time and you should not rush it. Plan to have someone with you for several days after having it done to ensure you are not overextending yourself. You will feel pain and discomfort, which is more like being sore. After the procedure, your doctor will want to see you within a few days to check on your progress.

The swelling and pain you feel will get less as the time goes by from the actual breast augmentation procedure. You can always talk to your doctor about what to expect by asking questions or looking at photos during your consultation.

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Friday, June 1, 2012

Marsha Temlock: ShouId I Encourage or Discourage My Kid to Get Divorced?

Some parents look on with horror when they realize their son or daughter's marriage is in trouble. Others are delighted that their unhappy child finally sees the light. The first scenario is more common, so I will begin by discussing parents who run to the rescue.

In response to an earlier blog post I wrote about the shock of receiving the divorce announcement, one parent commented: "We feel like observers of a train wreck. Our son recently decided to divorce our daughter-in-law after 11 years of marriage. They have 2 young children - ages 3 & 4 ... There were issues in their marriage that we found hard to understand; but our son seemed content, so we never commented on our observations. Then, for us, out of the blue, he wanted a divorce. At first we tried to 'fix' it, angering our son and then after many long distance conversations we accepted that it would happen."

It's hard to fault parents for wanting to fix what they perceive is broken. Some are willing to do everything in their power to keep the marriage afloat. They offer to pay for marriage counseling, to send the couple on a second honeymoon, to bail them out of financial difficulty, to set a jobless spouse up in business, even going so far as to buy the couple a larger house thinking a change in domicile will solve the problem.

For obvious reasons, some marriages should not be saved. Keep in mind, however, that parents are frequently in the dark and have no idea what is causing the breakup. Even if they know all the details, they should not take the lead unless their child is in danger. It's simply not the parent's decision to resolve or dissolve the marriage.

Butting out may be tough for a parent who has been divorced and thinks that his or her experience will help the adult child. No marriage is the same; no divorce is the same.

It's helpful to stop and wonder what motivates parents to get so involved. One embittered grandfather who advocated for his son's split spoke up in a parent support group to say that, to this day, he blamed his own parents for making him stick out his miserable marriage. "I stayed married eight years longer than I should have and it was hell."

A compelling reason to try to save the marriage has to do with grandchildren. "We've always been very close to our children and grandchildren and it is heartbreaking to have this happen and feel so helpless," wrote the parent who responded to my blog.

Unfortunately, grandparents often get the short end of the stick, but there are many things they can do to preserve the relationship. In my book Your Child's Divorce: What to Expect... What You Can Do? I suggest ways grandparents can strengthen the bonds with grandchildren at each stage of their parents' divorce.

It's natural to worry about the grandkids' well-being. Save the marriage, save the family. While there is a lot of evidence that children of divorce bear the scars into adulthood, today more and more children grow up in a single parent household. In their longitudinal study, Professor E. Mavis Hetherington and co-author John Kelley conducted a longitudinal study, For Better or For Worse, Divorce Reconsidered, observed 1,400 families and more than 2,500 children from divorced, intact and remarried families. They discovered that while there is a deleterious effect, the number of children who grew up, finished school, got jobs, formed healthy families and achieved impressive professional success were "uncommonly" resilient, mature, responsible and focused.

I truly believe that parents who interfere or take sides run the risk of alienating their child. There is no need to feel helpless; you can be someone your adult child can turn to when things look bleak. Be a trusted listener, a comforter, and -- if at all possible -- an optimist.

Your child's divorce is not the end of the world. Many parents think of it in terms of loss. Others who have watched their unhappy son or daughter from the sidelines call it a new beginning.

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US is heaping new pressure on Russia over Syria

COPENHAGEN, Denmark (AP) ? The U.S. is heaping new pressure on Russia to change course and support international action in Syria, warning that intransigence by Moscow may lead to open civil war that could spill across the Middle East with devastating effects.

Speaking on Russia's doorstep in Denmark, Secretary of State Hillary Rodham Clinton derided the Russian government for continuing to support Syrian President Bashar Assad, even after last week's massacre of more than 100 people in the town of Houla. In pointed remarks Thursday, she said Russia's position "is going to help contribute to a civil war" and rejected Russian officials' insistence that their stance actually is helping to ease the crisis.

On the first stop of a European tour, Clinton said Russia and China would have to be on board before the U.S. and other nations might engage in what could become a protracted conflict in support of a disorganized rebel force.

Russia, along with China, has twice vetoed U.N. Security Council sanctions against Syria. Russia is Syria's closest ally other than isolated Iran, and Clinton said that without its support the international community is essentially frozen from taking concrete steps to end the violence.

"The Russians keep telling us they want to do everything they can to avoid a civil war, because they believe that the violence would be catastrophic," Clinton said, noting that they are "vociferous in their claim that they are providing a stabilizing influence."

"I reject that," she said, complaining that in fact Russia is propping up Assad as his government continues a brutal, 15-month crackdown on dissent in which some 13,000 people have died.

A day earlier, White House Deputy National Security Adviser Denis McDonough said the U.S. is lobbying Russia to distance itself from its ally Syria and to apply pressure on Assad to leave office. A negotiated exit similar to one the U.S. helped broker for Yemen's longtime leader is one possibility, McDonough said, but he offered little optimism that the arguments are gaining traction.

The Obama administration has called on Assad to step down and clear the way for a political transition. But mindful of a war-weary American public, it has stopped short of advocating direct U.S. military engagement in the country and stressed the need for diplomacy, particularly with Moscow, which continues to sell weapons and provide political support to Assad.

In Washington, despite revulsion over the Houla massacre, officials said the administration remains deeply concerned about the possible consequences of military action. But as the violence continues, they acknowledge that planning for some sort of intervention is under way.

Two Western diplomats told The Associated Press that top U.S. and British commanders and intelligence officers visited the northern Jordanian city of Ramtha recently and toured the area along the Jordan-Syria border to assess the possibility of a limited land attack. But it was decided that air attacks would be best, considering the mostly bushy terrain and climate conditions, the diplomats said.

Still, Clinton told reporters after meeting top officials in Denmark, a key contributor to last year's NATO-led mission against Moammar Gadhafi in Libya that "we're nowhere near putting together any type of coalition other than to alleviate the suffering."

"We are working very hard to focus the efforts of those, like Denmark and the United States, who are appalled by what is going on, to win over those who still support the regime, both inside and outside of Syria."

At the White House, press secretary Jay Carney was blunt in describing the risks posed by the protracted standoff with Assad.

"The longer this goes on, the longer that Assad and his thugs are allowed to brutally murder the Syrian people, the more likely it becomes a sectarian civil war, the more likely that it spills over Syrian borders," Carney said. He listed another risk, of a "proxy war" with Iran backing Assad and other outside nations or forces backing insurgent factions.

The prospect of such a proxy war or a sectarian conflict along the lines of Lebanon is a major reason the United States is leery of arming the Syrian rebels or edging toward direct military involvement, though U.S. officials rarely say so.

Jordan has concerns about its territory, the Turks worry over Kurdish terrorists operating from inside Syria and neighboring Lebanon's is still struggling to emerge from decades of sectarian civil war and political instability.

Still, Moscow's calculus doesn't appear to be changing. Russia did begin inching away from Assad, particularly after the massacre in which dozens of women and children were among those shot at close range by pro-regime gunmen. And Foreign Minister Sergey Lavrov said this week that while both sides are to blame, Assad "bears the main responsibility for what is going on."

However, he also said the Houla massacre must not be a pretext to push for military intervention from outside. Instead, he urged all sides to focus on U.N. mediator Kofi Annan's peace plan, which by all accounts has failed to stem the violence.

Other than Russian opposition, there are other hurdles to armed intervention, U.S. officials said.

Clinton said Assad's foes lack the unity that the anti-Gadhafi camp eventually rallied in Libya. Syria's professional military and substantial air defenses also would make intervening far more difficult. And whereas in Libya the U.S. was able to count on the support of Gulf countries in monitoring a no-fly zone and carrying out some airstrikes, the Arab League is split on whether military options should be entertained in Syria.

"We know it could actually get much worse than it is," Clinton said. "We are trying to prevent that." Clinton underscored her support for the U.N. mediation effort, which she said had had some positive results even though none of its six points have been achieved. She stressed that U.N. observers have performed two important functions.

"In many of the areas where they are present, violence has gone down," Clinton said. "And they serve as independent observers, the eyes of the world if you will, in reporting back when terrible events like the recent massacre occur. They've tried to cut through the clutter and disinformation coming from the Syrian government."

She spoke as activists reported more shelling in the central Houla area.

The Britain-based Syrian Observatory for Human Rights and the Local Coordination Committees activist groups said Thursday's shelling of Houla was mostly by heavy machine guns. Survivors of last week's massacre blamed pro-regime gunmen for the close-range shooting of civilians in their homes, though the government denied the involvement of its troops.

Carney said the U.S. can't deal with Syria by itself.

"There is no question that as mighty as the United States is, that we cannot end all atrocities around the globe," Carney said.

The United States must approach world problems with an eye to its own national security interests and to what is possible to achieve, Carney said, while "making sure you're not taking actions that create unintended consequences that are bad for the United States and bad, in some cases, for the very people you're trying to help."

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Associated Press writers Anne Gearan in Washington and Jamal Halaby in Amman, Jordan, contributed to this report. Matthew Lee reported from Washington

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Man leaves jail, boards flight without ticket

A recently released prisoner managed to walk onto a flight in San Diego without having a ticket. KNSD-TV's Tony Shin reports.

By Sarah Grieco, Lauren Steussy and Tony Shin, NBC San Diego

A man released from jail on Memorial Day wound up boarding a plane without a ticket on Tuesday, San Diego Harbor Police said.

United Airlines flight 6323 was bound for Los Angeles from San Diego International Airport when flight attendants counted an extra passenger aboard the plane.


Authorities later learned the man entered the tarmac through an emergency door in the airport's commuter terminal. Although the alarm sounded, the man quickly made his way through the tarmac and boarded the plane with the other passengers.

A witness said he handed his duffel bag to a flight attendant, saying he urgently needed to use the bathroom. He then sat down in a passenger's seat.

The flight attendant realized there were too many people on the plane and identified the extra passenger. He was easy to spot, as he looked disheveled and suspicious, according to Nicholas Blasgen, a passenger on the plane.

Passengers were told to get off the plane while dogs sniffed their bags. They all were forced to go through security a second time before taking off.

"How could this happen?" Blasgen asked, "In this day and age when I have to take my shoes off to get on a plane, this guy bypasses everything. What if he had a bomb?"

The man was escorted off the plane peacefully. He was arrested and is now in jail. Harbor police are investigating the incident.

This story originally appeared on NBC San Diego.

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Arctic bacteria help in the search to find life on moon Europa

Wednesday, May 30, 2012

In a fjord in Canada scientists have found a landscape similar to one of Jupiter's icy moons: Europa. It consists of a frozen and sulphurous environment, where sulphur associated with Arctic bacteria offer clues for the upcoming missions in the search for traces of life on Europa.

It is not easy to find a place on Earth where ice and sulphur come together, supposedly like on Europa, Jupiter's moon. Nonetheless, this place has been located at Borup Fjord Pass in the Canadian High Arctic. Here the sulphurous yellow emissions contrast with the whiteness of the environment, creating images similar to those captured at Jupiter's satellite.

US researchers have now verified that the sulphur involved in the life cycle of Arctic microorganisms has some characteristics that could help to detect biological remains on Europa. Large space agencies like NASA and the European Space Agency are already in the process of preparing missions.

"We have discovered that elemental sulphur (S) can contain morphological, mineralogical and organic 'biosignatures' linked to bacterial activity. If they are found on Europa, this would suggest the possible presence of microorganisms," as explained to SINC by Damhnait Gleeson, lead author of the study and currently member of the Centro de Astrobiologia (INTA-CSIC, Spain).

The 'biosignatures' are associated with needle and rhomboid sulphur shapes in which mineralised remains of microorganisms and extracellular material appears. Thanks to electron microscopy and X-ray diffraction techniques, the formation of a rare type of sulphur has also been observed in association with organic components: the rosickyite. What is more is that small quantities (parts per million) of protein, fatty acids and other biomolecules have appeared in the sulphurous material.

"There is much evidence of bacterial activity," highlights Gleeson, who wonder if in the Europa's icy crust, or the ocean or lakes supposedly beneath it, there could be a similar microbial community that uses sulphur as their source of energy.

The researcher conducted the study, which has now been published in the Astrobiology journal, as a member of NASA's Jet Propulsion Laboratory and the University of Colorado in the USA. She is currently working as a scientist in the Centro de Astrobiologia in R?o Tinto (Spain), a place similar to Mars.

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FECYT - Spanish Foundation for Science and Technology: http://www.fecyt.es/fecyt/home.do

Thanks to FECYT - Spanish Foundation for Science and Technology for this article.

This press release was posted to serve as a topic for discussion. Please comment below. We try our best to only post press releases that are associated with peer reviewed scientific literature. Critical discussions of the research are appreciated. If you need help finding a link to the original article, please contact us on twitter or via e-mail.

This press release has been viewed 49 time(s).

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